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Image Search Results
Journal: Nucleic Acids Research
Article Title: Differential redox sensitivity of tRNA dihydrouridylation
doi: 10.1093/nar/gkae964
Figure Lengend Snippet: Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP C18 HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Article Snippet: The flow-through was incubated on 0.1 g
Techniques: Modification, Liquid Chromatography with Mass Spectroscopy, Isolation, Knock-Out, Negative Control, Comparison, Mass Spectrometry