silica gel 60 Search Results


96
MACHEREY NAGEL macherey nagel silica gel 230 400 mesh
Macherey Nagel Silica Gel 230 400 Mesh, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MACHEREY NAGEL silica gel
Silica Gel, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
MACHEREY NAGEL silica gel 60
Silica Gel 60, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MACHEREY NAGEL h2o meoh
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88
MACHEREY NAGEL polygoprep 60 50 c18 silica gel
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Polygoprep 60 50 C18 Silica Gel, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MACHEREY NAGEL polygoprep 60 30 nh2 lc packing material
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Polygoprep 60 30 Nh2 Lc Packing Material, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
MACHEREY NAGEL polygoprep 60 80
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Polygoprep 60 80, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
AK Scientific silica gel 60 230 mesh
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Silica Gel 60 230 Mesh, supplied by AK Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
EM Science Inc 230–400 mesh silica gel
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
230–400 Mesh Silica Gel, supplied by EM Science Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
HiMedia Laboratories silica gel 60
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Silica Gel 60, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Qualigen Inc silica gel 60–120 mesh
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Silica Gel 60–120 Mesh, supplied by Qualigen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Selecto Inc cellulose pei tlc sheets
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Cellulose Pei Tlc Sheets, supplied by Selecto Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP C18 HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.

Journal: Nucleic Acids Research

Article Title: Differential redox sensitivity of tRNA dihydrouridylation

doi: 10.1093/nar/gkae964

Figure Lengend Snippet: Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP C18 HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.

Article Snippet: The flow-through was incubated on 0.1 g POLYGOPREP 60–50 C18 silica gel (Macherey-Nagel) before being filtered through 3 kDa cut-off filters (Vivaspin, Merck) and subjected to LC–MS/MS measurement.

Techniques: Modification, Liquid Chromatography with Mass Spectroscopy, Isolation, Knock-Out, Negative Control, Comparison, Mass Spectrometry